MGF dose range
The amounts people commonly use, from the low end to the high end, with syringe units, schedule and half-life. The 9 published studies are one click away.
Animal studies only Growth hormone axis Also called mechano growth factor, IGF-1Ec, MGF C24E, MGF C25E, PEG-MGF
Common use ranges
From user guides and clinic write-ups. No drug label exists, and no study has tested these amounts in people. Community guides and one vendor dosing guide agree on about 200 mcg per shot for both forms. PEG-MGF upper amounts vary from 300 to 500 mcg.
- Half-life
- Not measured in any published study
- How it is taken
- Intramuscular into the trained muscle (MGF), subcutaneous (PEG-MGF)
- How long people use it
- 4 to 6 weeks for MGF, 4 to 8 weeks for PEG-MGF, then about 4 weeks off.
- Mixing used for the units
- 5 mg vial + 2 mL water. 1 unit on a U-100 insulin syringe = 25 mcg.
Pick your vial size and how much water you add. The units below change to match.
5 mg vial + 2 mL water: 1 unit = 25 mcg
| Tier | Each dose | Syringe units5 mg vial + 2 mL water | How often | Schedule | Vial lastsat this tier |
|---|---|---|---|---|---|
| Typical (MGF) [1] | 200 mcg | 8 units 0.08 mL | once a day | MGF: 100 to 200 mcg into the worked muscles within 30 to 60 minutes after training, on training days only | about 4 weeks |
| Starting (PEG-MGF) [2] | 100 mcg | 4 units 0.04 mL | 2x a week | PEG-MGF: 100 mcg 2 to 3 times a week | 6+ weeks |
| Typical (PEG-MGF) [3] | 200 mcg | 8 units 0.08 mL | 3x a week | PEG-MGF: 200 mcg 2 to 3 times a week, on rest days or after exercise | 6+ weeks |
| Higher (PEG-MGF) [4] | 400 mcg | 16 units 0.16 mL | 3x a week | PEG-MGF: 300 to 500 mcg 2 to 3 times a week | about 4 weeks |
Good to know
- Plain MGF lasts only about 5 to 7 minutes in the body, so it is injected right after training into the worked muscle.
- PEG-MGF lasts several hours, so it is taken a few times a week instead of every training day.
- There are no human dosing studies for MGF or PEG-MGF.
Sources for these amounts
- Typical (MGF): Peptide Protocol Wiki, MGF dosing (200 mcg post-workout, training days) · Peptide Protocol Wiki, PEG-MGF dosing (native MGF 100 to 200 mcg a day)
- Starting (PEG-MGF): Matrix Advanced Solutions, PEG-MGF dosing guide (starting dose)
- Typical (PEG-MGF): Peptide Protocol Wiki, PEG-MGF dosing (200 mcg 2 to 3 times weekly) · Peptide Protocol Wiki, MGF dosing (PEG-MGF 200 to 400 mcg 2 to 3 times weekly) · Matrix Advanced Solutions, PEG-MGF dosing guide (moderate dose)
- Higher (PEG-MGF): Matrix Advanced Solutions, PEG-MGF dosing guide (high dose 300 mcg) · Peptide Protocol Wiki, PEG-MGF dosing (200 to 500 mcg range)
How long people use it: Peptide Protocol Wiki, MGF dosing · Peptide Protocol Wiki, PEG-MGF dosing · Matrix Advanced Solutions, PEG-MGF dosing guide
Half-life: Mavrommatis et al, The E-domain region of mechano-growth factor inhibits cellular apoptosis and preserves cardiac function, Mol Cell Biochem 2013 · Dominikowski et al, The emerging landscape of performance-enhancing peptides modulating GH-IGF1 axis, Front Endocrinol 2026 (places PEG-MGF in the no-human-studies tier)
Take the MGF chart with you
Get a one-page printable chart for MGF, with the syringe units for each vial and water mix. We can also email you when a new MGF study is published.
This page shows what labels, clinics and users report, and what studies gave. It is not medical advice and not a recommendation for anyone. Many of these compounds are not approved for human use. Check your own medicines and lab work with a licensed clinician before using anything.
See the science: 9 published studies
No human dose data. No published human study of MGF states an amount given. Every figure on this page comes from animal studies.
Amounts given to animals in studies
Read this first. No human dosing study exists for MGF or PEG-MGF, and no animal dosing study of PEG-MGF is published. The mouse pump work used a chemically stabilised E-domain peptide, not the plain peptide.
Every study used for this page
Proof grade: Animal studies only. No human dose data has been published. Amounts are quoted as each study reported them. Where the abstract does not state an amount, the row says so.
| Study | Who was studied | Amount given, as reported | Route | How often | How long | What the authors reported |
|---|---|---|---|---|---|---|
| PMID 28599103Song et al, J Tissue Eng Regen Med 2018 | Rabbit, knee osteoarthritis model (group sizes not in abstract) | 0.1 to 10 mcgrams/mL (LOWEST verified) | Into the joint | not in abstract | 2 weeks | Held back cartilage breakdown. Cell work showed more proliferation and more matrix made |
| PMID 27334712Zhang et al, Biotechnol Lett 2016 | Rat, cut Achilles tendon (group sizes not in abstract) | 1000 ng/mL of MGF-C25E | Into the tendon site | not in abstract | not in abstract | Better tendon function score and stiffer repaired tendon than saline |
| PMID 31164836Liu et al, Front Physiol 2019 | Mouse, bruised calf muscle with macrophages removed (group sizes not in abstract) | 5 mcgrams in 20 microlitres per muscle | Into the muscle | day 0, day 1 and day 2 after injury | 3 doses | Did not rescue muscle fibre regrowth but cut scarring and lowered inflammatory and matrix-breaking genes. Dose read from PMC6534059 full text |
| PMID 41272763Moro et al, Arthritis Res Ther 2025 | Rat, HLA-B27 and human beta2m transgenic, arthritis model, n=6 per group | 3 microlitres/kg and 10 microlitres/kg (concentration not given, so no mass dose) | not in abstract | every 3 days | 7 weeks | Delayed arthritis onset from 14 to 23 days at the high dose. Raised IL-2 and IL-10, lowered TNF-alpha, STAT3, ROR-gamma-t and IL-17. Doses read from PMC12639975 full text |
| PMID 23712705Mavrommatis et al, Mol Cell Biochem 2013 | Mouse, heart attack model, n not in abstract | 4.5 mg/kg per day | Under the skin by pump | continuous | 2 weeks, pump started 12 h before the heart attack | Kept heart contractility, held back overgrowth and left fewer dying cells. Dose read from PMC3720995 full text |
| PMID 25606570Shioura et al, Int J Cardiovasc Res 2014 | Mouse, heart attack model, n=9 per group | 4.5 mg/kg per day (HIGHEST verified) | Under the skin by pump | continuous | 2 weeks, started either within 12 h or at 8 weeks after the heart attack | Both timings improved heart and vessel function at 10 weeks. The later start prevented decompensation. Dose read from PMC4297642 full text |
| PMID 27623796Chen et al, Biotechnol Lett 2016 | Rat, 14 day tail suspension to mimic weightlessness (group sizes not in abstract) | Not stated in the abstract | Into the muscle | not in abstract | 14 days | Reversed the rise in memory-linked genes and microRNAs in the hippocampus |
| PMID 34015701Sha et al, Int Immunopharmacol 2021 | Rat, monosodium iodoacetate osteoarthritis (group sizes not in abstract) | Not stated in the abstract | not in abstract | not in abstract | 4 and 6 weeks | Slowed cartilage loss and raised type II collagen |
| PMID 42395176Dominikowski et al, Front Endocrinol 2026 | review, no subjects | n/a | n/a | n/a | n/a | Places PEG-MGF and IGF-1 LR3 in the tier with no human studies at all, and warns that online products have unknown content and dose |
How to read the study numbers
- One study, one group. Each figure is what one team chose for its own participants or animals.
- Route matters. An amount given into a vein (IV) is not the same as the same amount under the skin, in the nose or by mouth.
- Animals are not small people. An animal amount is shown as the study gave it. It is not a human amount.
- Short studies. Many studies ran days or weeks. They do not show what happens over longer use.
Common questions
Is there a human dose for MGF?
No published human study of MGF states an amount given. The figures on this page come from animal studies only.
Where do these MGF numbers come from?
From 9 published studies, each linked to its PubMed or ClinicalTrials.gov record. Every figure was checked against the study abstract or its open full text. Proof grade: Animal studies only.
How long does MGF stay in the body?
No published study has measured the half-life of MGF, so nobody knows exactly how long one dose stays in the blood.
Is this a recommendation for how much MGF to use?
No. This page shows the amounts reported by drug labels, clinics and users, next to what researchers gave in studies. It is not medical advice and not a recommendation for anyone. Talk to a licensed clinician before using any of it.