Humanin dose range
The amounts people commonly use, from the low end to the high end, with syringe units, schedule and half-life. The 8 published studies are one click away.
Animal studies only Mitochondrial Also called HN, S14G-humanin, HNG
Common use ranges
From user guides and clinic write-ups. No drug label exists, and no study has tested these amounts in people. Only community and education sites give amounts, and they agree on roughly 1 to 3 mg two or three times a week. They differ on whether that amount refers to native humanin or the much stronger HNG form.
- Half-life
- about 30 minutes in mice, over 4 hours in rats
- How it is taken
- Subcutaneous injection
- How long people use it
- 8 to 12 weeks on, then a 4 to 6 week break. Some guides use a shorter 4 to 8 weeks on, 2 to 4 weeks off.
- Mixing used for the units
- 15 mg vial + 2 mL water. 1 unit on a U-100 insulin syringe = 75 mcg.
Pick your vial size and how much water you add. The units below change to match.
15 mg vial + 2 mL water: 1 unit = 75 mcg
| Tier | Each dose | Syringe units15 mg vial + 2 mL water | How often | Schedule | Vial lastsat this tier |
|---|---|---|---|---|---|
| Starting [1] | 1 mg | 13.3 units 0.13 mL | 2x a week | 1 mg twice a week | 6+ weeks |
| Typical [2] | 2 mg | 26.7 units 0.27 mL | 3x a week | 1 to 3 mg per shot, 2 to 3 times a week | 17 days |
| Higher [3] | 3 mg | 40 units 0.40 mL | 3x a week | 5 to 10 mg in total each week, split into 2 to 3 shots | 11 days |
Good to know
- There is no approved human dose for humanin.
- HNG, a modified form of humanin, is reported to be about 1000 times stronger, and the HNG range quoted in guides is 100 to 500 mcg. Check which form a vial holds before comparing amounts.
- A low daily option of 0.5 to 1 mg once a day for 4 to 8 weeks also appears in community guides.
Sources for these amounts
- Starting: Outliyr, Humanin report (conservative native humanin, 1 mg twice weekly)
- Typical: Outliyr, Humanin report (standard 1 to 3 mg, 2 to 3 times weekly) · Peptide Protocol Wiki, Humanin community protocols (1 to 5 mg, 2 to 3 times weekly)
- Higher: Peptide Protocol Wiki, Humanin community protocols (higher-dose weekly) · Peptide Protocol Wiki, Humanin dosing calculator
How long people use it: Outliyr, Humanin report · Peptide Protocol Wiki, Humanin community protocols
Take the Humanin chart with you
Get a one-page printable chart for Humanin, with the syringe units for each vial and water mix. We can also email you when a new Humanin study is published.
This page shows what labels, clinics and users report, and what studies gave. It is not medical advice and not a recommendation for anyone. Many of these compounds are not approved for human use. Check your own medicines and lab work with a licensed clinician before using anything.
See the science: 8 published studies
No human dose data. No published human study of Humanin states an amount given. Every figure on this page comes from animal studies.
Amounts given to animals in studies
Read this first. Nearly all data use the analogue S14G-humanin (HNG), not native humanin, and no person has been given any amount.
Every study used for this page
Proof grade: Animal studies only. No human dose data has been published. Amounts are quoted as each study reported them. Where the abstract does not state an amount, the row says so.
| Study | Who was studied | Amount given, as reported | Route | How often | How long | What the authors reported |
|---|---|---|---|---|---|---|
| PMID 28726291Thummasorn et al, Cardiovasc Ther 2017 | Rat, male Wistar, heart ischemia and reperfusion (group sizes not in abstract) | 84, 168 and 252 mcgrams/kg (LOWEST verified) | Into the vein | one time, 15 min after ischemia started and again at reperfusion | single study day | Only the 252 mcgrams/kg dose cut arrhythmia, infarct size and heart muscle failure |
| PMID 29376862Kumfu et al, J Alzheimers Dis 2018 | Rat, male Wistar, n=36 total (30 ischemia, 6 sham) | 84, 168 and 252 mcgrams/kg | Into the vein | one time during ischemia, or at the start of reperfusion | single study day | 168 and 252 mcgrams/kg protected brain mitochondria and lowered tau and amyloid markers |
| PMID 31172328Mehta et al, Metabolomics 2019 | Mouse, diet-induced obese, n=6 per group | 2.5 mg/kg per injection | Into the belly | twice a day | 3 days | Shifted plasma glutathione and sphingolipid metabolites, in line with insulin sensitising |
| PMID 23836030Chin et al, Endocrinology 2013 | Mouse (wild type and IGFBP-3 knockout) and adult male Sprague Dawley rat | 2 mg/kg in mice, 5 mg/kg in rats (per methods) | Into the belly | one time | single dose, blood sampled out to 24 h | Humanin half-life about 30 min in mice and over 4 h in rats. Highest levels in plasma, some in liver, none found in brain or heart |
| PMID 29775411Lue et al, Am J Physiol Heart Circ Physiol 2018 | Mouse, adult male, n=80 across 8 groups | 5 mg/kg | Into the belly | once a day | 10 weeks | Added to dexrazoxane, protected the heart against doxorubicin damage |
| PMID 30004252Qin et al, Am J Physiol Heart Circ Physiol 2018 | Mouse, female C57BL/6N, old group n=6, treated n=8, young control n=5 | 4 mg/kg (longest run) | Into the belly | 2 times a week | 14 months, from 18 to 32 months of age | Less heart scarring and less cell death in very old mice |
| PMID 26384090Lue et al, Endocrinology 2015 | Mouse, young adult male, melanoma model (group sizes not in abstract) | Not stated in the abstract | Into the belly, and under the skin in the second arm | once a day | 3 weeks, then a 4 week arm | Protected sperm stem cells and white cells during chemotherapy and still cut lung spread |
| PMID 26058861Gong et al, Am J Physiol Endocrinol Metab 2015 | Mouse, high fat diet fed (n not in abstract) | Not stated in the abstract | Into the belly, plus vein and brain arms for the acute work | repeated (schedule not in abstract) | not stated | Less weight gain, less belly fat and less liver fat. The liver fat effect ran through the brain and the vagus nerve |
How to read the study numbers
- One study, one group. Each figure is what one team chose for its own participants or animals.
- Route matters. An amount given into a vein (IV) is not the same as the same amount under the skin, in the nose or by mouth.
- Animals are not small people. An animal amount is shown as the study gave it. It is not a human amount.
- Short studies. Many studies ran days or weeks. They do not show what happens over longer use.
Common questions
Is there a human dose for Humanin?
No published human study of Humanin states an amount given. The figures on this page come from animal studies only.
Where do these Humanin numbers come from?
From 8 published studies, each linked to its PubMed or ClinicalTrials.gov record. Every figure was checked against the study abstract or its open full text. Proof grade: Animal studies only.
How long does Humanin stay in the body?
Its half-life is about 30 minutes in mice, over 4 hours in rats, measured in animals, not people. Half-life is how long it takes for half of a dose to leave the blood. The effect can last longer than that.
Is this a recommendation for how much Humanin to use?
No. This page shows the amounts reported by drug labels, clinics and users, next to what researchers gave in studies. It is not medical advice and not a recommendation for anyone. Talk to a licensed clinician before using any of it.